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Rose Scientific
4027 - 97 Street Edmonton,
Alberta, Canada T6E 5Y5
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Rose Scientific
4027 - 97 Street Edmonton,
Alberta, Canada T6E 5Y5
  Telephone:
Toll free:
Fax:
  780.438.5110
800.661.9289

780.462.5776
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Biomass Sensors, Hamilton Incyte Capacitance Viable Cell Density and Dencytee Optical Density Total Cell Density

  Overview

The Hamilton Incyte viable cell density measurement is based on capacitance. Viable cells behave like little capacitors and their polarization and depolarization in an alternating electrical field is measured. The signal can be correlated to the bio-volume. Only viable cells can be polarized in the alternating electrical field so this method is insensitive to cell debris as well as to microcarriers.

A measuring loop consists of an Incyte sensor that is available in different dimensions, an Incyte pre-amlifier and an Arc View controller. The Arc View can display both parameters at once using the 2 (Arc View 265) or 4 (Arc View 465, 465 XL) channel inputs. Additionally Arc pH and DO sensors with a wireless module can be displayed on the controller giving the ability to see all relevant parameters of a cell culture or fermentation on one screen. The Incyte sensor and pre-amplifier are sold as a calibrated Incyte Unit but are also available as spare parts.

The Hamilton Dencytee total cell density measurement is based on the optical density of a suspension at NIR wavelengths. All particles and molecules that scatter the NIR light will be detected and can be correlated to the total cell density. The Dencytee sensor keeps the light intensity at the detector constant leading to a wide linearity range.

A measuring loop consists of a Dencytee sensor with an optical path length of 5 mm, a Dencytee pre-amplifier and the Arc View controller. The controller can display both parameters at once using the 2 (Arc View 265) or 4 (Arc View 465, 465 XL) channel inputs. Additionally Arc pH and DO sensors with a wireless module can be displayed on the controller giving the ability to see all relevant parameters of a cell culture or fermentation on one screen. The Denytee sensor and pre-amplifier are sold as a Dencytee Unit but are also available as spare parts.

Features of the Incyte and Dencytee Cell Density Sensors:
  • The capacitance based Incyte cell density sensors give detail insight into cell culture and fermentation
  • Capacitance sensors are insensitive to micro-carriers, gas bubbles, cell debris and other particles in suspension
  • The Dencytee optical density sensors are based on turbidity, or optical density, measured at Near Infra-Red Wavelengths (NIR)
  • Sold as a Unit, consisting of an Incyte or Dencytee sensor and an individual pre-amplifier
  • An Arc View Controller is required, to display two parameters at once, using the 2 channel (Arc View 265) or 4 channel (Arc View 465, 465XL) inputs
  • Additional Arc pH and DO sensors with a wireless module can be used with an Arc View controller to see all relevant parameters of a cell culture or fermentation on one screen
Specifications:  Biomass Sensors, Hamilton Incyte Capacitance Viable Cell Density and Dencytee Optical Density Total Cell Density

NumberDescriptionPrice $
243712 Incyte Viable Cell Density Unit DN25 – DG BE Capacitance Sensor, 54 mm length
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243711 Incyte Viable Cell Density Unit DN25 – DG Capacitance Sensor, 52 mm length
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243710 Incyte Viable Cell Density Unit DN25 – SG Capacitance Sensor, 72 mm length
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243700 Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 120 mm length, 12 mm Ø
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243701 Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 220 mm length, 12 mm Ø
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243702 Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 320 mm length, 12 mm Ø
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243703 Incyte Viable Cell Density Unit DN12 Capacitance Sensor, 420 mm length, 12 mm Ø
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243755 Dencytee Total Cell Density Unit DN12-120 Optical Density Sensor, 120 mm length, 12 mm Ø
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243756 Dencytee Total Cell Density Unit DN12-220 Optical Density Sensor, 220 mm length, 12 mm Ø
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243757 Dencytee Total Cell Density Unit DN12-320 Optical Density Sensor, 420 mm length, 12 mm Ø
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243758 Dencytee Total Cell Density Unit DN12-420 Optical Density Sensor, 420 mm length, 12 mm Ø
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243800 Arc View 265 Controller to collect and record data generated by Incyte and Dencytee sensors, wireless, must be equipped with proper license (243822, 243823, 243824, 243820), Data Input/Output RS485, AUX to Analog Output Box, Digital outputs Modbus RTU (RS485), Ethernet connection with RJ45 (OPC XML-DA), USB for downloading data or firmware upgrade, 2 measuring channels, 0-80% operating humidity, -10 to 50°C operating temperature range, stainless steel housing, W×D×H = 250×220×110 mm
Inquire
243801 Arc View 465 Controller to collect and record data generated by Incyte and Dencytee sensors, wireless, must be equipped with proper license (243822, 243823, 243824, 243820), Data Input/Output RS485, AUX to Analog Output Box, Digital outputs Modbus RTU (RS485), Ethernet connection with RJ45 (OPC XML-DA), USB for downloading data or firmware upgrade, 4 measuring channels, 0-80% operating humidity, -10 to 50°C operating temperature range, stainless steel housing, W×D×H = 250×220×110 mm
Inquire
243802 Arc View 465XL Controller to collect and record data generated by Incyte and Dencytee sensors, wireless, must be equipped with proper license (243822, 243823, 243824, 243820), Data Input/Output RS485, AUX to Analog Output Box, Digital outputs Modbus RTU (RS485), Ethernet connection with RJ45 (OPC XML-DA), USB for downloading data or firmware upgrade, 4 measuring channels, 0-80% operating humidity, -10 to 50°C operating temperature range, stainless steel housing, W×D×H = 443×114×364 mm
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243810 PC AUX to Analog Output Box, 4 measuring channels, display information on a computer, USB input and output
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243873 Sensor cable, 40 m, M12.5 Pole male/female process connection
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243872 Sensor cable, 20 m, M12.5 Pole male/female process connection
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243871 Sensor cable, 10 m, M12.5 Pole male/female process connection
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243870 Sensor cable, 5 m, M12.5 Pole male/female process connection
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243842 Solution B for Incyte Calibrations, 180 mL sodium sulphate solution for 2 calibrations from 2 - 50 mS
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243740 Validation and Calibration Kit for Incyte Sensors, includes Solution B (243842), Standard conductivity solution 12,85 mS and Calibrator to check the internal electronics of the Incyte Pre-Aplifier
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238988 Conductivity Standard, 12880 μS/cm, Basic Line, Accuracy ± 1%, Stability 18 months, Value at 25°C 12880 µS/cm, 500 mL Calpack bottle
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243850 Analog Output Box, transfer measurements from Arc View Controller to the Process Control System, 4 outputs for Cell Density, 4 outputs for Conductivity
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243852 Cable for Analog Outputs Box, 10 m. M12 5 Pole / DIN-RAIL process connection
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243851 Cable for Analog Output Box, 5 m. M12 5 Pole / DIN-RAIL process connection
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243822 Incyte License required to use Incyte sensors with ARC Controllers Inquire
243823 Incyte Scan License for optional measurement at 17 additional frequencies to analyze a bioprocess in more detail Inquire
243824 Dencytec License required to use Dencyte sensors with ARC Controllers Inquire
243820 OPC XML-CA LicenseInquire


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