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Rose Scientific
4027 - 97 Street Edmonton,
Alberta, Canada T6E 5Y5
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Rose Scientific
4027 - 97 Street Edmonton,
Alberta, Canada T6E 5Y5
  Telephone:
Toll free:
Fax:
  780.438.5110
800.661.9289

780.462.5776
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Send by Email to: sales@rosesci.com
Print and Fax to: 780.462.5776
 

Columns, SMT O Series Octyl SAM C8 HPLC

  Overview

SMT’s unique bonding method produces very high ligand density silica based octyl (C8) column packing material that results in chromatographic properties unlike any other silica-based C8 column support.

SMT’s C8 column is designed for optimum peak shape, peak capacity and high chromatographic efficiency for both acidic and basic solutes.

SMT’s C8 columns are very stable at extreme pH conditions and high temperatures. The column is strongly recommended for the separation of most basic solutes that have high concentrations of very hydrophobic functional groups. C8 column is usually the second column of choice after C18 for method developments using reversed-phase chromatographic separation. When compared to other columns such as C4, CN, phenyl, or an amino bonded phase, the C8 is the most hydrophobic.

Although C18 remains the most widely used, the C8 phase represents a good compromise phase. C8 phase normally provides equivalent selectivity; it is not too hydrophobic, and yet it retains many compounds on the basis of interaction with their hydrophobic groups. C8 phases are good choices when too much organic solvent is required to elute the analytes of interest (especially highly hydrophobic molecules) from a C18 phase. The use of C8 columns reduces retention time and consumption of organic solvents

SMT O Series is the SAM-C8 phase with the highest functional ligand coverage confirmed with carbon analysis results of 12% carbon load. In this series, a very high density of the functional ligand, octyl molecule or C8, is achieved through meticulous mixture of the C8 with proprietary spacer molecules to ensure maximum coverage. These phases are very hydrophobic and are designed to tolerate usage in mildly aggressive pH conditions and high temperatures. SMT SAM-C8 columns are designed to withstand a pH range of 1-10. The columns are generally 50% more stable than those of competitors used under similar conditions.

Features of SMT O Series Octyl SAM C8:
  • Moderately hydrophobic, designed to tolerate usage in mildly aggressive pH conditions
  • Highly versatile; offers selectivity for polar and moderately nonpolar pharmaceuticals, natural products, organic chemicals and biologicals
  • Particle sizes of 3, 5 and 10 µm are stock, others available
  • Pore sizes of 100 and 300Å are stock, others available

A comparison of hydrophobicity in C8 versus C18 columns in the analysis of nonpolar compounds.

Nonpolar compounds C8:
Column: O-5-100-15
Solutes: 1 = uracil
2 = benzene
3 = toluene
4 = naphthalene
5 = iso-butylbenzene
6 = anthracene
Eluent: ACN: Water 60:40 (v:v)
Flow: 1.0 mL/min
Detector: UV; 254nm
Temp: 30° C
Remarks: C8 usually offers similar selectivities as C18 but with reduced retention of hydrophobic analytes.

Nonpolar compounds C18:
Column: OD-5-100-15
Solutes: 1 = uracil
2 = benzene
3 = toluene
4 = naphthalene
5 = iso-butylbenzene
6 = anthracene
Eluent: ACN: Water 60:40 (v:v)
Flow: 1.0 mL/min
Detector: UV; 254nm
Temp: 30° C
Remarks: Note the change in selectivities of iso-butyl benzene and anthracene when SMT SAM-C8 is compared with SAM-C18 under similar chromatographic conditions.


Specifications:  Columns, SMT O Series Octyl SAM C8 HPLC

NumberDescriptionPrice $
O-3-100-2 SMTSAM-C8 O Column, 3 µm, 100Å, 20 mm L x 4.6 mm I.D565.00
O-3-100-3 SMTSAM-C8 O Column, 3 µm, 100Å, 30 mm L x 4.6 mm I.D592.65
O-3-100-5 SMTSAM-C8 O Column, 3 µm, 100Å, 50 mm L x 4.6 mm I.D619.65
O-3-100-7.5 SMTSAM-C8 O Column, 3 µm, 100Å, 75 mm L x 4.6 mm I.D646.65
O-3-100-10 SMTSAM-C8 O Column, 3 µm, 100Å, 100 mm L x 4.6 mm I.D673.65
O-3-100-15 SMTSAM-C8 O Column, 3 µm, 100Å, 150 mm L x 4.6 mm I.D700.65
O-3-100-25 SMTSAM-C8 O Column, 3 µm, 100Å, 250 mm L x 4.6 mm I.D781.65
O-3-120-2 SMTSAM-C8 O Column, 3 µm, 120Å, 20 mm L x 4.6 mm I.D565.00
O-3-120-3 SMTSAM-C8 O Column, 3 µm, 120Å, 30 mm L x 4.6 mm I.D592.65
O-3-120-5 SMTSAM-C8 O Column, 3 µm, 120Å, 50 mm L x 4.6 mm I.D619.65
O-3-120-7.5 SMTSAM-C8 O Column, 3 µm, 120Å, 75 mm L x 4.6 mm I.D646.65
O-3-120-10 SMTSAM-C8 O Column, 3 µm, 120Å, 100 mm L x 4.6 mm I.D673.65
O-3-120-15 SMTSAM-C8 O Column, 3 µm, 120Å, 150 mm L x 4.6 mm I.D700.65
O-3-120-25 SMTSAM-C8 O Column, 3 µm, 1020Å, 250 mm L x 4.6 mm I.D781.65
O-5-100-02 SMTSAM-C8 O Column, 5 µm, 100Å, 20 mm L x 4.6 mm I.D498.15
O-5-100-03 SMTSAM-C8 O Column, 5 µm, 100Å, 30 mm L x 4.6 mm I.D511.65
O-5-100-05 SMTSAM-C8 O Column, 5 µm, 100Å, 50 mm L x 4.6 mm I.D525.15
O-5-100-7.5 SMTSAM-C8 O Column, 5 µm, 100Å, 75 mm L x 4.6 mm I.D565.65
O-5-100-10 SMTSAM-C8 O Column, 5 µm, 100Å, 100 mm L x 4.6 mm I.D592.65
O-5-100-15 SMTSAM-C8 O Column, 5 µm, 100Å, 150 mm L x 4.6 mm I.D660.15
O-5-100-25 SMTSAM-C8 O Column, 5 µm, 100Å, 250 mm L x 4.6 mm I.D700.65
O-5-100-30 SMTSAM-C8 O Column, 5 µm, 100Å, 300 mm L x 4.6 mm I.D795.15
O-10-100-05 SMTSAM-C8 O Column, 10 µm, 100Å, 50 mm L x 4.6 mm I.D552.15
O-10-100-7.5 SMTSAM-C8 O Column, 10 µm, 100Å, 75 mm L x 4.6 mm I.D592.65
O-10-100-10 SMTSAM-C8 O Column, 10 µm, 100Å, 100 mm L x 4.6 mm I.D619.65
O-10-100-15 SMTSAM-C8 O Column, 10 µm, 100Å, 150 mm L x 4.6 mm I.D646.65
O-10-100-25 SMTSAM-C8 O Column, 10 µm, 100Å, 250 mm L x 4.6 mm I.D687.15
O-10-100-30 SMTSAM-C8 O Column, 10 µm, 100Å, 300 mm L x 4.6 mm I.D781.65
O-5-300-02 SMTSAM-C8 O Column, 5 µm, 300Å, 20 mm L x 4.6 mm I.D498.15
O-5-300-3 SMTSAM-C8 O Column, 5 µm, 300Å, 30 mm L x 4.6 mm I.D511.65
O-5-300-05 SMTSAM-C8 O Column, 5 µm, 300Å, 50 mm L x 4.6 mm I.D525.15
O-5-300-7.5 SMTSAM-C8 O Column, 5 µm, 300Å, 75 mm L x 4.6 mm I.D565.65
O-5-300-10 SMTSAM-C8 O Column, 5 µm, 300Å, 100 mm L x 4.6 mm I.D592.65
O-5-300-15 SMTSAM-C8 O Column, 5 µm, 300Å, 150 mm L x 4.6 mm I.D646.65
O-5-300-25 SMTSAM-C8 O Column, 5 µm, 300Å, 250 mm L x 4.6 mm I.D687.15
O-5-300-30 SMTSAM-C8 O Column, 5 µm, 300Å, 300 mm L x 4.6 mm I.D781.65
O-10-300-05 SMTSAM-C8 O Column, 10 µm, 300Å, 50 mm L x 4.6 mm I.D552.15
O-10-300-7.5 SMTSAM-C8 O Column, 10 µm, 300Å, 75 mm L x 4.6 mm I.D592.65
O-10-300-10 SMTSAM-C8 O Column, 10 µm, 300Å, 100 mm L x 4.6 mm I.D619.65
O-10-300-15 SMTSAM-C8 O Column, 10 µm, 300Å, 150 mm L x 4.6 mm I.D646.65
O-10-300-25 SMTSAM-C8 O Column, 10 µm, 300Å, 250 mm L x 4.6 mm I.D687.15
O-10-300-30 SMTSAM-C8 O Column, 10 µm, 300Å, 300 mm L x 4.6 mm I.D781.65
O-5-60-2 SMTSAM-C8 O Column, 5 µm, 60Å, 20 mm L x 4.6 mm I.D498.15
O-5-60-3 SMTSAM-C8 O Column, 5 µm, 60Å, 30 mm L x 4.6 mm I.D511.65
O-5-60-5 SMTSAM-C8 O Column, 5 µm, 60Å, 50 mm L x 4.6 mm I.D525.15
O-5-60-7.5 SMTSAM-C8 O Column, 5 µm, 60Å, 75 mm L x 4.6 mm I.D565.65
O-5-60-10 SMTSAM-C8 O Column, 5 µm, 60Å, 100 mm L x 4.6 mm I.D592.65
O-5-60-15 SMTSAM-C8 O Column, 5 µm, 60Å, 150 mm L x 4.6 mm I.D660.15
O-5-60-25 SMTSAM-C8 O Column, 5 µm, 60Å, 250 mm L x 4.6 mm I.D700.65
O-5-60-30 SMTSAM-C8 O Column, 5 µm, 60Å, 300 mm L x 4.6 mm I.D795.15


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